CBD testimonial

I am 53 years old and I am suffering from multiple sclerosis since December 1995. I treatment "Betaferon" for 6 ½ years.

This two and a half years, I saw a program on the therapeutic use of cannabis and tired of swallowing too much medication, I tried ...

At the time, I re-walked modestly (100 meters) helped a cane, otherwise wheelchair ...

I tried a first time smoking: the effects on the pain is undeniable.

However, I noticed a "better" without it being fully satisfied.

So I fumbled to get a decoction in oil, one tablespoon morning and evening for one month, the results were different: I felt able to manage the pain differently.

So I went on a protocol decoction smoking and the results obtained, I did not change the treatment.

This 22 months, you could see on the MRI as plates had become inactive (ie myelin is destroyed not more), but nevertheless remained active plates where the disease continued to progress.

Since July 2001, I normally walk again, it was difficult at first, I had remuscler me and lose weight (I was 125 pounds).

I have no cane or wheelchair and I resumed a normal occupation (I'm multimedia trainer).

On MRI, passed in September, 2002, we can see that no more plate is active and that the right side of the brain, some components of myelin are replenished. The radiologist, thinking that his machine was faulty or wanton, carried out a second time tests: the results are there and there!

In two years, I have made tremendous progress, I no longer have to walk imbalance, I do not manage the intensity of the pain, but its appearance. I have a new life

source ;http://www.positifs.org/old/temoins/medicaux/tm3d.htm

QUANTITATIVE ANALYSIS OF CANNABIS OIL Part two: method

This method uses the Thin Layer Chromatography (TLC)

for material and reagents .. see part one

Cut the sheet to the required size white side up (be careful, the silica layer is fragile, do not touch it with your fingers)

Two centimeters (1 inch) from the bottom edge, draw a light line with a pencil, draw on this line marks all minimum 2cm (fit based on test samples) see Figure 1 - Phase 1

Identify the plate so no questionable (date, batch reference) drive the top edge (the first centimeters)

Figure 1

Activation of the plate:

By heating at 100 ° C (212 ° F) for one hour (domestic oven)

Choice of the compound of reference:

In the absence of pure products (difficult to obtain regard to the legislation)

Take a lot that has been tested by an outside laboratory, reserve a part and keep in the freezer) them split in aliquots of + / - 100mg

Preparation of the samples:

Weigh accurately about 10 mg of the test product (oil)

Dissolve in 20 ml of ethanol to obtain a concentration of 0.5mg/ml

Adjust the volume of ethanol by weighing (eg, weighing 10.5 mg = 21 ml of ethanol

Using a capillary tube gently lay one µl of solution (0.5µg substance).  Does not make craters

Successively 1μLde the reference solution (spot A), 1 μL of test solution (spot B) 2μL of the test solution (spot C)….. (see Figure 1)

USE  ONE CAPILLARY BY A SINGLE SPOT!

allow to dry

Preparation of the mobile phase (eluent)

Petroleum ether 60/90 (CAS 8032-32-4) ........ 80% v / v

Di-ethyl ether (CAS: 60-29-7.) ...................... 20% v / v

The volume to prepare depends on the tank used, typically 50 ml is sufficient (40 ml petroleum ether 60/90 and 10 ml of diethyl ether)

  Prepare a strip of absorbent paper (towel) with a width equal to a major surface of the tank (or two fifths diameter if it is a round tank) See Figure 2

Figure 2

Pour  the mobile phase in the tank, cover and let stand 15 minutes to saturate

Place the test plate in the tank (the side of the base line in the eluent)

the solvent does NOT reach the baseline

Close the tank

The migration time depends on the temperature (not working at too high temperature)

Remove the plate when the solvent front reaches approximately 2.5 cm (1 inch) from the top edge of the plate

.

The dry a few minutes with a hairdryer

Revelation

Prepare 10 ml of a solution composed of:

Fast blue B salt (CAS: 5486-84-0 / C.I. 37175) .... 25 mg

0.1N NaOH (CAS 1310-73-2) ........................... 10 mL

Spray lightly and evenly on the plate (do not soak)

let it dry

The color is stable over time. For added security, a photo can be done, take care to place a measuring stick in the field of the photo (further measures maintaining the scale)

Using a pencil,

Leading the front line of solvent

Wrap each spot as accurately as possible to conclusively identify (a1, a2, a3 .... B1 b2 b3 ...)

Mark the center

Measure and record the following:

The distance between the baseline and the solvent front  D1(Figure 1)

The distance between the baseline and the center of each spot  D 2 (Fig. 1)

References :

A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L..  - Issue 5 - 2009 - pp. 421-426Justin T. Fischedick - Ronald Glas - Arno Hazekamp - Rob Verpoorte - Phytochemical Analysis - Vol. 20

 F. Geiss, Die Parameter der Dünnschicht-Chromatographie, Verlag F. Vieweg & Sohn, Braunschweig, 1972

Méthodes recommandées pour l’identification et l’analyse du cannabis et des produits du cannabis, Section scientifique et du laboratoire, UNITED NATIONS OFFICE ON DRUGS AND CRIME Vienna 2010

QUANTITATIVE ANALYSIS OF CANNABIS OIL part one , Material and reagents

A simple, reliable method of analysis, cheap,
Accessible to everyone without special chemical knowledge

Reusable material  

Glass tank  (#usable models)

Test tubes

1-5-10 ml pipettes and Graduated test tube

spay

 

Balance

Pencil -  measuring stick – cutter- label

  

Disposables

Aluminum foil SG60 20x20 cm   box of 25  ref : Merck-5553-7 ( to realize 50 test)

Paper towels- Gloves

Chemical reagents

Petroleum ether 60/90 (CAS 8032-32-4)

Diethyl ether (CAS: 60-29-7).

NaOH 0.1N - sodium hydroxide (CAS 1310-73-2)

Fast blue B salt (CAS: 5486-84-0 / CI 37175)

Water

Make Cannabis Oil .... the choice of Solvent

What solvent to produce oil?

For the manufacture of cannabis oil, many solvents are used.

Before choosing a solvent, it should be borne in mind that our manufacture a DRUG to be used, absorbed by sick people who trust. must work as a professional, the amateur must be outlawed

It is our responsibility to develop a quality product, to proceed with the utmost care. Should therefore be selected appropriate materials! The quality of the finished product shall be equal to the quality of the worst ingredient.

Be certain than the right  product is used: several totally different products can have the same trade  name (danger of confusion) only guaranteed when a trade name is given, always associate the No. CAS International

The C.A.S (Chemical Abstract Service) is an identifier determined by computer. The algorithm identifies structural diagrams and automatically allocates a CAS unique to each chemical entity (molecule, mixture of isomers, industrial product).

This issue is divided into three parts, separated by dashes. It is therefore of the form: YYYYYY-XX-X with Y: 3 to 6 digits.

Given the complexity of chemical nomenclature and the possibility to identify a substance by several names, CAS number identifies the chemical species unambiguously.

You can find this number tablespoon in the Chemical Substance Index: http://www.cas.org/

Naphtha

The Naphtha: petroleum, which is a generic name for a mixture of compounds boiling variable (140 to 400 ° F) aliphatic alkanes and paraffins used as paint thinner. Naphtha sold in drugstores is a technical product containing impurities

To be avoided as such

Petroleum ether

CAS Number 101316-46-5

Petroleum ether is refined naphtha with a boiling point are known, it consists of a mixture of aliphatic alkanes (eg pentane, hexane etc. ...)

One can easily find three grades of petroleum ether (Grade: pure, with analysis report)

A-fraction distilled between 90 and 220 °F (the most interesting for oil)

B- Fraction distilled between 220 and 250 ° F

C-Fraction distilled between 250 and 300 ° F

Advantage: chlorophyll is insoluble

Disadvantages: smell residual oil in low concentration (an odorless oil is a guarantee of complete removal of the solvent)

This is probably the product that is used for Simpson oil, medical grade

Hexane

CAS Number 110-54-3

Hexane is a constituent of petroleum ether, it is a pure product with stable and defined physico-chemical characteristics (specific boiling point)

Advantage: pure product

Disadvantages: very expensive

Ethyl alcohol

CAS Number 64-17-5

The most common solvents!

However, there are a wide variety of qualities (from vodka .. until absolute alcohol)

It is difficult to obtain absolute alcohol (99%) it absorbs water from the air to form a stable mixture 96%

Vodka (50%) is not suitable .. it contains too much water and requires heat above 100 degrees to eliminate (degradation CBD)

Warning: alcohol for disinfection same undenatured is dosed at 70% (to prevent coagulation of proteins)

Denatured alcohol by means of sulfuric ether is dangerous: in addition to its characteristic odor is unstable and degrades explosive peroxides hightly hepatotoxic

Advantages: non-toxic trough levels, relatively easy to obtain

Disadvantage: chlorophylls dissolved, giving a dark green color and a special flavor to the oil.

Isopropyl alcohol

CAS Number 67-63-0

Identical to ethyl alcohol Features

Used for disinfection (70%)

Undenatured

Butane

Lighter gas,

Used only for very small quantities (some grams of raw material)

EXPLOSIVE!

Advantage: no evaporation to make

Disadvantage : obligation to work outside, Explosive, heavier than air

Coconut Oil

CAS Number 8001-31-8

Used as an extraction solvent, it does not allow high concentration, concentration is impossible, because there is no evatoration

This is the ideal vehicle to dilute the oil extracted by other methods, and allow a constant dosage of active ingredients

Corn oil

CAS Number 8001-30-7

As for coconut oil

Make Oil.....What is my goal: THC or CBD?

What is my goal: TCH or CBD?

This is the first question that must be answered!

The final therapeutic use depends on it!

If the goal is to treat nausea, pain, muscle spasms, stimulate appetite....THC should be preferred

In this case, extract up a temperature of 250 ° F  is tolerated

The ideal is to work at the lowest possible temperature.

The choice of solvent is critical!

                           

Priority should be given to CBD and other active compounds if you want to treat other diseases such as convulsions cancer, autoimmune diseases, blood circulation, anxiety .. and many others (see chart above)

it is imperative to carry extraction at low temperature, because at 160 ° F THCA begins to degrade to transform itself into THC

All compounds of cannabis have a melting point lover than 160 ° F (except THC)

Beyond this temperature they begin to be partially eliminated with the solvent during the final phase of evaporation

it is a fundamental principle in all extraction processes with plant material:

LOW EXTRACTION TEMPERATURE

= THE BETTER THE FINISHED PRODUCT

= THE BETTER THE CONSERVATION

REFLECTIONS ABOUT CANNABIS AND ITS LEGISLATION

We live in times both difficult and exciting!

In Europe, bit of countries are adopted specific legislation except Portugal and Holland. Many consider the possession and cultivation how a very low priority and do not criminalize.

In the United States, twenty states have passed legislation .. more or less restrictive.

The medical cannabis (medical marijuana) occupies a unique position.

In the light of all these changing laws appears a fact: The cannabis, use, distribution, and traditional culture are in a late reign

A new era was born: the industrialization of the market, the past decade has been rich in therapeutic discoveries  about cannabis .. The pharmaceuticals companies are interested, with their lobbyists and their 'suggestion' of laws… for the profits

Consider the case of Michigan in 2008, citizens voted in favor (63%) for medical marijuana (MMM act) a binding law but overall little restrictive (next federal laws). The patient can legally grow their medicine, or buy from a caregiver who render a service. the patient has been registered through annual payment to the state. The patient rewards contingent caregiver for this service.

In reality: Being caregiver has become a profession! The concept of service is missing the selling price between $ 5 and $ 10 a gram for a production cost lower than $ 1 per gram and free of specific tax (4% as tax on all sales of food products ) barely cheaper than the black market prices.

overview of taxe level state by states

 In general, patients do not have significant financial resources (no health insurance, unemployed person / precarious work, no access to medical care, cannabis is their only medicine) and make important financial efforts to obtain their drug)

When a caregiver responds to a patient (who told him that its treatment was costing him $ 1,500 monthly): I have a family to feed, I have to pay my home, my health insurance, my retirement plan, education of my children , my car etc. .. Where is the concept of service desired by the citizens in 2008 MMMact? where is the compassion?

Five years after the government wants to legislate and impose more stringent conditions for the caregiver AND the patient! Only the pharmaceutical companies will continue their business ... on the backs of patients (one month treatment Sativex is equal to $ 300)

Even with an equivalent tax on tobacco, the sale price should be at about twice a pack of cigarettes ($ 10 for 20 cigarettes)

medical marijuana brings in $10m_for_michigan

http://www.theawl.com/2012/06/pack-of-cigarettes-cost

Echinacea: a natural interferon ?

Common name: purple coneflower.

Botanical names: Echinacea angustifolia, E. pallida, E. purpurea, family Asteraceae.

English names: Echinacea, American cone flower, snakeroot.

Parts used: Roots (E. pallida, E. angustifolia, E. purpurea) and aerial parts (E. purpurea).

Habitat and origin: all species of coneflowers are native to North America. Three of them have the same medicinal value. Natural stands of Echinacea mainly colonized the American Great Plains without crossing the Canadian border to the north, or to reach the Mexican border in the south. Today, they are cultivated in all temperate climates, in sunny locations where the soil is rich and well drained.

History:

Echinacea has a long and intriguing history of use. For hundreds of years, the Plains Indians used it as an antiseptic, an analgesic, and to treat poisonous insect and snakebites, toothaches, sore throat, wounds and communicable diseases such as mumps, smallpox, and measles. It was also used by the Cheyenne, Choctaw, Comanche, Dakota, Meskawaki Fox, Pawnee, Sioux, and Omaha tribes.  It was used as a treatment for saddle sores on horses. Echinacea became known in Europe around 1895. Many research studies done by doctors in Germany indicated that echinacea is effective primarily by increasing the number of white blood cells, thus boosting the immune system and thereby increasing the body's ability to fight infections.

active substances

Alkaloids indolizidiniques derived amino acid and have a nitrogen atom in a heterocyclic ring system.

Indolizidiniques alkaloids derived from L-lysine and have a core feature of the indolizidine.

Castanospermine and swansonine acids are indoliziniques.

Properties indoliziniques alkaloids are:

Inhibition of proliferation and tumor dissemination

immunomodulatory action

Stimulating the production of interleukin-2 and T cell proliferation

Activity against retroviruses (HIV), due to the ability to interfere with the functions of the envelope glycoprotein of the virus.

Action against cytomegalovirus (CMV)

Echinacea is so effective because it actually mimics the actions of a chemical the human body produces called interferon, which basically shields cells from viral infection. As an anti-viral, the herb is capable of building-up the body's immune system according to German researchers, by increasing the amount of T-cells, white-blood cells in the body that fight infection, by 30 percent. An article by the University of Pittsburgh called "About Echinacea" goes into further detail about Echinacea A. healing properties, stating that it contains many chemical components such as essential oils, Vitamin B1, B2, and B3, kaempferol, and terpenoids just to name a few, in addition to the natural anti-biotics echinacoside and echinacein, that all allow the plant to neutralize enzymes that invade cells and attack healthy tissues. Echinacea has very complex molecules whose structure is very similar to what is found on the surface of bacteria or other microbes. It is believed that echinacea simulates microbial invasion and alert the entire immune system! It is a bit like an alarm,

Preventing leukopenia caused by radiotherapy or chemotherapy. In the late 1980s, German researchers launched a series of preliminary clinical trials aimed at determining if a preparation containing extracts of Echinacea, wild indigo and white cedar could minimize the adverse effects of radiotherapy, particularly for what is the drop in white blood cells (leukopenia).
Echinacea can also be used externally. Although not normally available commercially, injections and topical use can be helpful in improving skin conditions like herpes lesions, sunburn, eczema and wounds.

Physical Action: Summary

  Traditionally used by American Indians to fight infections (colds, tonsillitis ...), biting snakes, cutannees diseases, intestinal pain, etc.. Today its use is limited mainly to the stimulation of the immune system to treat and prevent infections of upper and lower respiratory tract and other viral, bacterial or fungal infections. It is very popular in Germany and is approved as a treatment support during respiratory infections or urinary tract. Fungal infections as Candida albicans and Listeria, monocytogenes, chronic fatigue syndrome, chronic arthritis, cancer, chronic pelvic infections, etc.. It is also used in topical form in Europe in order to accelerate wound healings due to injuries, burns, herpes or inflammatory disorders (eczema. ..)

Where to buy : http://botanicalguides.com/echinaceapurpurea.html

How use?

Infusion. : Infuse for 10 minutes, 1 g of roots or dried aerial parts in 1 cup of boiling water. Drink from 1 to 6 per day.

Decoction : Boil for 5 to 10 minutes, 1 g of roots of Echinacea in 1 cup of water. Take up to three cups per day.

Capsules.: Containing only powder capsules for roots or aerial parts, it is recommended to take the equivalent of 1 g, 3 times a day.

NOTE: A report on mayo clinic  suggests that people with autoimmune diseases should consult a physician before using this plant. Be careful with duration of use as a treatment too long, it can cause immuno-suppressant (inversion effect)

References:

Schapowal A, Berger D, et al. Eur J Med Res. 2009 Sep 1;14(9):406-12. Echinacea/sage or chlorhexidine/lidocaine for treating acute sore throats: a randomized double-blind trial. 

 Linde K, Barrett B, et al. Echinacea for preventing and treating the common cold.Cochrane Database Syst Rev. 2006 Jan 25;(1):CD000530.

Cohen HA, Varsano I, et al. Effectiveness of an herbal preparation containing echinacea, propolis, and vitamin C in preventing respiratory tract infections in children: a randomized, double-blind, placebo-controlled, multicenter study.Arch Pediatr Adolesc Med. 2004 Mar;158(3):217-21.

Pochernyayeva VF. Echinacea symposium presents new research on Chernobyl victims. 1999 (June); International Echinacea Symposium (Kansas City), American Herbal Products Association.

Bendel R, Bendel V, et al. [Additional treatment with Esberitox N in patients with chemo-radiotherapy treatment of advanced breast cancer].  Onkologie 1989 Jun;12 Suppl 3:32-8. 

Freeman C, Spelman K. A critical evaluation of drug interactions with Echinacea spp. Mol Nutr Food Res. 2008 Jul;52(7):789-98. Review.